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KMID : 0377519980230020085
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Chung-Ang Journal of Medicine
1998 Volume.23 No. 2 p.85 ~ p.91
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Comparison of reverse transcriptase-polymerase chain reaction methods for detection of. hepatitis C virus RNA
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Lee Mi-Kyung
Park Ae-Ja
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Abstract
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Diagnosis for the hepatitis C virus(HCV) infection currently is based on the detection of anti-HCV antibodies or HCV RNA. The HCV RNA in the patient’s sera can detected in the early acute infection stage before antibody appearance or aminotransferase elevation and from the chronic patients who failed to produce antibodies. This technology, however, has not been standadized well and is laborious and expensive. Two HCV detection kits(A and B) were compared with HCV reverse transcriptase-polymerase chain reaction(RT-PCR) method of Choun-Ang University Hospital to establish the most sensitive and relaible method for HCV RNA detection. We tested 30 HCV RNA positive and 10 HCV RNA negative sera. Agreement with three HCV RT-PCR methods was 82.5%. In using HCV detection kits, the reverse transcription and first round PCR step are carried out in a single tube. The lesser manipulations reduced risks of contamination and labour. But the two kits appeared not to be improved in sensitivity and reproducibility of the HCV RT-PCR test.
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KEYWORD
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Hepatitis C virus, reverse transcriptase-polymerase chain reaction
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